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Thrust 1 Leads
Thrust Area TA-1: Studying Bio-Nitrogen Fixation in SD Natural Ecosystems
The Northern Plains host a variety of environments in which to seek N2-fixing bacteria and thereby enrich our knowledge of them. In past years, with a small local funding support, we have isolated more than ten N2- fixing strains from natural ecosystems in SD. In this E-RISE project, we will continue to identify N2-fixing microbes that can function in oxic environments and/or with some fixed N present, paying special attention to those that may be useful in agriculture and biomanufacturing. Upon grant renewal, in years 5-7 we will extend Project description: BioNitrogen Economy Research Center (BNERC) this work to diverse natural ecosystems across the US, in particular partnering with other land grant and tribal institutions to survey N-fixers from a wide variety of native ecosystems including indigenous food and medicinal plants in other ecoregions.
TA-1.1: Identify N2-fixing cyanobacteria from natural ecosystems and conduct genome sequencing
TA-1.2: Bioprospecting of thermophilic N2-fixing cyanobacteria/bacteria
TA-1.3: Characterization of novel N2-fixers associated with plants and nif gene mining
TA-1.4: Bioprospecting of native rhizobia for competitive colonization of field pea
TA-1.5: Machine learning-driven comparative genomics to identify genes important in N-fixation
Identified Sampling Site at SD
Thrust 2 Leads
Thrust Area-2 (TA-2): Comparative Multi-omics Study of Light-dependent, Oxic N2-fixation
The Northern Plains host a variety of environments in which to seek N2-fixing bacteria and thereby enrich our knowledge of them. In past years, with a small local funding support, we have isolated more than ten N2- fixing strains from natural ecosystems in SD. In this E-RISE project, we will continue to identify N2-fixing microbes that can function in oxic environments and/or with some fixed N present, paying special attention to those that may be useful in agriculture and biomanufacturing. Upon grant renewal, in years 5-7 we will extend Project description: BioNitrogen Economy Research Center (BNERC) this work to diverse natural ecosystems across the US, in particular partnering with other land grant and tribal institutions to survey N-fixers from a wide variety of native ecosystems including indigenous food and medicinal plants in other ecoregions.
TA-1.1: Identify N2-fixing cyanobacteria from natural ecosystems and conduct genome sequencing
TA-1.2: Bioprospecting of thermophilic N2-fixing cyanobacteria/bacteria
TA-1.3: Characterization of novel N2-fixers associated with plants and nif gene mining
TA-1.4: Bioprospecting of native rhizobia for competitive colonization of field pea
TA-1.5: Machine learning-driven comparative genomics to identify genes important in N-fixation
Identified Sampling Site at SD
Thrust 3 Leads
Thrust Area-3 (TA-3): BNF-driven Biomanufacturing
This thrust area is focused on using genetically engineered N2-fixing cyanobacteria to produce N-rich bioproducts including biofertilizer (ammonia), quality nutritional proteins, and clean fuel hydrogen (H2).
TA-3.1: Biomass from N2-fixing cyanobacteria as N-fertilizer
TA-3.2: Engineering of N2-fixing cyanobacteria to produce nutritional proteins for fish meal
TA-3.3: Biomanufacturing Hydrogen (H2) using thermophilic N2-fixing cyanobacteria
TA-3.4 Socioeconomic Impact
Identified Sampling Site at SD
Thrust 4 Leads
Thrust Area-4 (TA-4): Grafting of Biological Nitrogen-Fixation
TA-4.1: Genomic grafting: ML-generated minimal genome transformation
TA-4.2: Plant grafting: grafting symbiotic N2-fixation machinery to plants
Identified Sampling Site at SD
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